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Direct determination of lactulose in heat‐treated milk using diffuse reflectance infrared Fourier transform spectroscopy and partial least squares regression
Author(s) -
Pappas Christos S,
Sakkas Lambros,
Moschopoulou Ekaterini,
Moatsou Golfo
Publication year - 2015
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12233
Subject(s) - partial least squares regression , lactulose , diffuse reflectance infrared fourier transform , chemistry , diffuse reflection , analytical chemistry (journal) , fourier transform infrared spectroscopy , fourier transform , infrared spectroscopy , infrared , spectroscopy , linear regression , second derivative , chromatography , mathematics , optics , statistics , physics , organic chemistry , mathematical analysis , biochemistry , photocatalysis , quantum mechanics , catalysis
The aim of this work was the direct determination of lactulose ( LCT ) concentration in freeze‐dried heat‐treated milks using diffuse reflectance Fourier transform infrared spectroscopy ( DRIFTS ) without any chemical pretreatment of the milk. The ‘actual’ lactulose concentrations estimated by means of high‐performance liquid chromatography ( HPLC ) were correlated with the spectral region 1286–754 cm −1 of DRIFTS spectra in the second‐derivative form of the milk samples using partial least squares ( PLS ) regression. A linear relationship was established between the ‘actual’ and the concentrations recalculated using the built model. The proposed DRIFTS method is simple, rapid and low cost.