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Effect of different matrices on probiotic resistance to in vitro simulated gastrointestinal conditions
Author(s) -
Casarotti Sabri,
Todorov Svetoslav D,
Penna Ana Lúcia B
Publication year - 2015
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12215
Subject(s) - probiotic , bifidobacterium animalis , inulin , lactobacillus acidophilus , food science , gastrointestinal tract , in vitro , microbiology and biotechnology , biology , chemistry , bacteria , lactobacillus , fermentation , biochemistry , bifidobacterium , genetics
In this study, we evaluated the effect of different matrices ( MRS , milk and milk with inulin) on the tolerance of probiotic strains ( L actobacillus acidophilus L a‐5 and B ifidobacterium animalis subsp. lactis BB ‐12) to simulated conditions similar to those found in the gastrointestinal tract. Both probiotic strains demonstrated a significantly lower viability after exposure to in vitro gastric and intestinal conditions, and B . animalis subsp. lactis BB ‐12 showed higher survival during the test compared to L . acidophilus L a‐5 in all tested matrices. Milk and inulin protected probiotics from in vitro gastrointestinal stress. These results suggest that it is critical to formulate the food matrix to be used as probiotic carrier.