Premium
Determining N eu5 A c in infant formula with ultra‐performance liquid chromatography–tandem mass spectrometry
Author(s) -
Li Chun,
Liu Libo,
Xie Honglei,
Liu Ning
Publication year - 2015
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12204
Subject(s) - chromatography , detection limit , chemistry , trichloroacetic acid , tandem mass spectrometry , sialic acid , mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , infant formula , biochemistry
The aim of this work was to measure N ‐acetylneuraminic acid ( N eu5 A c) in milk‐based infant formulas. The analysis was performed by ultra‐performance liquid chromatography–tandem mass spectrometry ( UPLC ‐ MS / MS ). The total N eu5 A c were released using trichloroacetic acid and hydrochloric acid and purified using a HLB column. The linearity from 0.05 to 5.0 μg/mg N eu5 A c was adequate. Sialic acid recoveries ranged from 91.8% to 112.4%. The detection and quantification limits (limit of detection, 0.01 μg N eu5 A c/mg; limit of quantitation, 1.08 μg N eu5 A c/mg) were low enough to determine the sialic acid in infant formulas. The validated method is highly reproducible and sensitive, and it is easy to perform.