Fluorescent immunoassay for determination of penicillin and sulphonamide residues in milk using carboxylic magnetic nanoparticles | Zendy

Ivanova Svetla | Zendy; Gabrovska Katya | Zendy; Godjevargova Tzonka | Zendy

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Fluorescent immunoassay for determination of penicillin and sulphonamide residues in milk using carboxylic magnetic nanoparticles

Author(s) -

Ivanova Svetla,

Gabrovska Katya,

Godjevargova Tzonka

Publication year - 2014

Publication title -

international journal of dairy technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.061

H-Index - 53

eISSN - 1471-0307

pISSN - 1364-727X

DOI - 10.1111/1471-0307.12172

Subject(s) - immunoassay , chromatography , chemistry , penicillin , fluorescence , nanoparticle , antibody , antibiotics , biochemistry , materials science , nanotechnology , biology , physics , quantum mechanics , immunology

A fluorescent immunoassay for separate and simultaneous determination of penicillin ( PEN ) and sulphadimethoxine ( SDM ) in milk was developed. Monoclonal antibodies immobilised on carboxylic magnetic nanoparticles ( MNP s) were used. It was proved that the protein A ‐oriented immobilisation method ( OI ) provided a 1.5‐times more sensitive assay than the random immobilisation one ( RI ). The activation of MNP s with a mixture of N ‐(3‐dimethylaminopropyl)‐ N ‐ethylcarbodiimide ( EDC ) and N ‐hydroxysuccinimide ( NHS ) ensured a five‐times higher sensitivity of immunoassay than the activation using EDC . The linearity of standard curves in milk was: 3/10 ng/mL PEN ( OI and RI ), 50/250 ng/mL SDM ( OI ), and 50/500 ng/mL SDM ( RI ).

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