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Κ‐ CN gene polymorphism in Nili‐ravi buffalo, Achai and Sahiwal cattle of Pakistan
Author(s) -
ghafoor Abdul,
Riaz Muhammad N,
Zahur Aamer B,
Abbas Nasir,
Yousaf Muhammad,
Shah Abdullah,
Ishaq Rafaqat,
Suleman Muhammad
Publication year - 2015
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12146
Subject(s) - genotype , biology , polymorphism (computer science) , restriction fragment length polymorphism , casein , gene , dairy cattle , genetics , polymerase chain reaction , genotype frequency , veterinary medicine , zoology , food science , medicine
Kappa‐casein (κ‐ CN ) is the subtype of casein protein, an important constituent of bovine milk protein. The current study was undertaken to investigate the genetic polymorphism in κ ‐ CN gene of Nili‐ravi buffalo, Achai and Sahiwal cattle of Pakistan using polymerase chain reaction–restriction fragment length polymorphism ( PCR ‐ RFLP ) technique. The Nili‐ravi buffalo was found to be monomorphic (genotype BB only) for κ ‐ CN gene. Achai cattle were polymorphic for κ ‐ CN (having three genotypes AA , AB and BB ) with a frequency of 0.70, 0.18 and 0.12, respectively, while in Sahiwal cattle, both the genotypes AA and AB were found with genotypic frequencies of 0.92 and 0.08, respectively. The presence of genotype BB in Achai cattle is surprising as it is absent in most of the cattle breeds worldwide.