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Investigation of the ability of dairy nonstarter lactic acid bacteria to grow using cell lysates of other lactic acid bacteria as the exclusive source of nutrients
Author(s) -
Sgarbi Elisa,
Bottari Benedetta,
Gatti Monica,
Neviani Erasmo
Publication year - 2014
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12132
Subject(s) - lactic acid , lysis , bacteria , lactobacillus casei , lactobacillus rhamnosus , lactobacillus helveticus , food science , biology , lactococcus lactis , lactobacillus , microbiology and biotechnology , chemistry , biochemistry , genetics
A new device named a cell sonicated substrate membrane system ( CSSMS ), consisting of a tissue culture insert that supplies nutrients, in the form of starter lactic acid bacteria ( SLAB ) lysate extract, through a polycarbonate membrane which isolates it from the nonstarter lactic acid bacteria ( NSLAB ) inoculum and serves as a support for bacterial growth, was developed. The CSSMS was used to demonstrate that the Lactobacillus helveticus SLAB lysate provides sufficient nutrients to allow the growth of L. casei and L. rhamnosus NSLAB in vitro . The results support the hypothesis that NSLAB could grow in aged cheese using only SLAB cell lysate as nutrients.