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Autolysis detection and evaluation of some lactic acid bacteria by renaturing sodium dodecyl sulphate‐polyacrylamide gel electrophoresis and polymerase chain reaction assays
Author(s) -
AlSaleh Abdulrahman A,
Ismail Elsayed A,
Metwalli Ali Am
Publication year - 2014
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12113
Subject(s) - autolysis (biology) , polymerase chain reaction , biology , gel electrophoresis , bacteria , sodium dodecyl sulfate , lactobacillus , polyacrylamide gel electrophoresis , lysogenic cycle , microbiology and biotechnology , chemistry , biochemistry , enzyme , gene , escherichia coli , bacteriophage , genetics
Eleven lactic acid bacterial strains were tested for autolysis ability and the presence of autolytic enzymes by renaturing sodium dodecyl sulphate‐polyacrylamide gel electrophoresis ( SDS ‐ PAGE ). Polymerase chain reaction ( PCR ) assays for the detection of lysogenic strains were performed. Autolysis in a buffer system was observed in L actobacillus delbrueckii subsp. bulgaricus DSM 20080 , L. delbrueckii subsp. bulgaricus DSM 20081 , B ifidobacterium longum subsp . infantis DSM 20088 , B. angulatum DSM 20098 and S treptococcus thermophillus DSM 20617. Mitomycin C induction of prophage was demonstrated in B. longum subsp. infantis DSM 20088 , B. angulatum DSM 20098, L actobacillus acidophilus DSM 20242 and S. thermophillus DSM 20617. The presence of genes encoding known bacteriophage lysins was demonstrated by a PCR assay and correlated well with the autolytic phenotypes of the strains, indicating that PCR screening is useful in the rapid identification of autolytic strains.