Premium
Detection of progesterone in bovine milk using an electrochemical immunosensor
Author(s) -
Zhang Hong You,
Du Xia Yan,
Liu Qian,
Xia Cheng,
Sun Ling Wei
Publication year - 2013
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12076
Subject(s) - detection limit , coefficient of variation , estriol , chromatography , linear range , chemistry , ovulation , biochemistry , hormone
To improve the detection of ovulation and early diagnosis of pregnancy on dairy farms, a disposable electrochemical immunosensor was developed using indirect competitive immunoassays and screen‐printed carbon electrodes. After optimisation of the assay conditions, the linear range was from 0.16 to 50 ng/ mL . The limit of detection was 0.16 ng/ mL . There was no cross‐reactivity with testosterone, 17‐β‐estradiol and estriol. The intra‐assay coefficient of variation was less than 13% and interassay coefficient of variation was < 9%. The percentage recovery was from 95.8% to 115.7%. Stability lasts 4 days at 4 °C. The results of preliminary application trials indicate that the method could have practical potential.