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Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system
Author(s) -
Shen Xi,
Wang Bingxin,
Yang Nianda,
Zhang Lulu,
Shen Danyu,
Wu Huijun,
Dong Ying,
Niu Ben,
Chou ShanHo,
Puopolo Gerardo,
Fan Jiaqin,
Qian Guoliang
Publication year - 2021
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.15662
Subject(s) - biology , bacteria , secretion , microbiology and biotechnology , type three secretion system , microbiome , pathogenic bacteria , gene , mutant , genetics , biochemistry
Summary Soil microbiome comprises numerous microbial species that continuously interact with each other. Among the modes of diverse interactions, cell–cell killing may play a key role in shaping the microbiome composition. Bacteria deploy various secretion systems to fend off other microorganisms and Type IV Secretion System (T4SS) in pathogenic bacteria was shown to function as a contact‐dependent, inter‐bacterial killing system only recently. The present study investigated the role played by T4SS in the killing behaviour of the soilborne biocontrol bacterium Lysobacter enzymogenes OH11. Results showed that L . enzymogenes OH11 genome encompasses genes encoding all the components of T4SS and effectors potentially involved in inter‐bacterial killing system. Generation of knock‐out mutants revealed that L . enzymogenes OH11 uses T4SS as the main contact‐dependent weapon against other soilborne bacteria. The T4SS‐mediated killing behaviour of L . enzymogenes OH11 decreased the antibacterial and antifungal activity of two Pseudomonas spp. but at the same time, protected carrot from infection by Pectobacterium carotovorum . Overall, this study showed for the first time the involvement of T4SS in the killing behaviour of L . enzymogenes and its impact on the multiple interactions occurring in the soil microbiome.