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Comparison of mitochondrial genomes provides insights into intron dynamics and evolution in Botryosphaeria dothidea and B . kuwatsukai
Author(s) -
Wang Bo,
Liang Xiaofei,
Hao Xiaojuan,
Dang Haiyue,
Hsiang Tom,
Gleason Mark L.,
Zhang Rong,
Sun Guangyu
Publication year - 2021
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.15608
Subject(s) - botryosphaeria dothidea , biology , orfs , genetics , intron , genome , phylogenetic tree , botany , evolutionary biology , gene , open reading frame , peptide sequence
Summary Botryosphaeria dothidea is one of the most common fungal pathogens on a large number of hosts worldwide. Botryosphaeria dothidea and B. kuwatsukai are also the main causal agents of apple ring rot. In this study, we sequenced, assembled and annotated the circular mitogenomes of 12 diverse B . dothidea isolates (105.7–114.8 kb) infecting various plants including apple, and five diverse B . kuwatsukai isolates (118.0–124.6 kb) from apple. B . dothidea mitogenomes harboured a set of 29–31 introns and 48–52 ORFs. In contrast, B . kuwatsukai mitogenomes harboured more introns (32–34) and ORFs (51–54). The variation in mitogenome sizes was associated mainly with different numbers of introns and insertions of mobile genetic elements. Interestingly, B . dothidea and B . kuwatsukai displayed distinct intron distribution patterns, with three intron loci showing presence/absence dynamics in each species. Large numbers of introns (57% in B . dothidea and 49% in B . kuwatsukai ) were most likely obtained through horizontal transfer from non‐Dothideomycetes. The mitochondrial gene phylogeny supported the differentiation of the two species. Overall, this study sheds light into the mitochondrial evolution of the plant pathogens B . dothidea and B . kuwatsukai , and intron distribution patterns could be useful markers for studies on population diversity.