Interconnection of the mycobacterial heparin‐binding hemagglutinin with cholesterol degradation and heme/iron pathways identified by proximity‐dependent biotin identification in Mycobacterium smegmatis

Summary Deciphering protein–protein interactions is a critical step in the identification and the understanding of biological mechanisms deployed by pathogenic bacteria. The development of in vivo technologies to characterize these interactions is still in its infancy, especially for bacteria whose subcellular organization is particularly complex, such as mycobacteria. In this work, we used the proximity‐dependent biotin identification (BioID) to define the mycobacterial heparin‐binding hemagglutinin (HbhA) interactome in the saprophytic bacterium Mycobacterium smegmatis . M . smegmatis is a commonly used model to study and characterize the physiology of pathogenic mycobacteria, such as Mycobacterium tuberculosis . Here, we adapted the BioID technology to in vivo protein–protein interactions studies in M . smegmatis , which presents several advantages, such as maintaining the complex organization of the mycomembrane, offering the possibility to study membrane or cell wall‐associated proteins, including HbhA, in the presence of cofactors and post‐translational modifications, such as the complex methylation pattern of HbhA. Using this technology, we found that HbhA is interconnected with cholesterol degradation and heme/iron pathways. These results are in line with previous studies showing the dual localization of HbhA, associated with the cell wall and intracytoplasmic lipid inclusions, and its induction under high iron growth conditions.