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RNA release via membrane vesicles in Pseudomonas aeruginosa PAO1 is associated with the growth phase
Author(s) -
PérezCruz Carla,
Briansó Ferran,
Sonnleitner Elisabeth,
Bläsi Udo,
Mercadé Elena
Publication year - 2021
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.15436
Subject(s) - rna , biology , pseudomonas aeruginosa , exponential growth , vesicle , microbiology and biotechnology , transcriptome , stationary phase , bacterial growth , messenger rna , phase (matter) , membrane , biophysics , gene expression , bacteria , biochemistry , gene , chromatography , chemistry , genetics , mathematical analysis , mathematics , organic chemistry
Summary Pseudomonas aeruginosa PAO1 membrane vesicles (MVs) are known to play a role in cell‐to‐cell communication. Several studies have shown that the MV composition and physicochemical properties vary according to the bacterial growth stage, but the impact this might have on the externalization of RNA via MVs has not been addressed. Therefore, a study to characterize the RNA content from MVs retrieved at different growth phases was conducted. First, the transcriptome analyses revealed a higher abundance of around 300 RNA species in MVs when compared with the cells. The vesiculation rate along the growth curve was determined, showing that the release of MVs increased during the transition to the stationary phase, whereas it decreased in the late stationary phase. RNA‐seq of MVs retrieved along the transition to the stationary phase demonstrated that the RNA cargo of vesicles did not vary. However, the amount of smaller RNAs (<200 nt) inside MVs retrieved in the late exponential phase was higher than in the stationary phase MVs. These results indicate that the externalization of RNA via MVs occurs during late exponential phase and implies the secretion of different types of MVs during growth.