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Comparative proteomics of Geobacter sulfurreducens PCA T in response to acetate, formate and/or hydrogen as electron donor
Author(s) -
Mollaei Monir,
Timmers Peer H. A.,
SuarezDiez Maria,
Boeren Sjef,
Gelder Antonie H.,
Stams Alfons J. M.,
Plugge Caroline M.
Publication year - 2021
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.15311
Subject(s) - geobacter sulfurreducens , formate , formate dehydrogenase , electron acceptor , citric acid cycle , electron donor , metabolic pathway , chemistry , biochemistry , biology , inorganic chemistry , metabolism , bacteria , catalysis , biofilm , genetics
Summary Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled to the reduction of Fe(III). The response of G. sulfurreducens to the electron donors acetate, formate, hydrogen and a mixture of all three with Fe(III) citrate as electron acceptor was studied using comparative physiological and proteomic approaches. Variations in the supplied electron donors resulted in differential abundance of proteins involved in the citric acid cycle (CAC), gluconeogenesis, electron transport, and hydrogenases and formate dehydrogenase. Our results provided new insights into the electron donor metabolism of G. sulfurreducens . Remarkably, formate was the preferred electron donor compared to acetate, hydrogen, or acetate plus hydrogen. When hydrogen was the electron donor, formate was formed, which was associated with a high abundance of formate dehydrogenase. Notably, abundant proteins of two CO 2 fixation pathways (acetyl‐CoA pathway and the reversed oxidative CAC) corroborated chemolithoautotrophic growth of G. sulfurreducens with formate or hydrogen and CO 2 , and provided novel insight into chemolithoautotrophic growth of G. sulfurreducens .