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Role of enterotoxigenic Escherichia coli prophage in spreading antibiotic resistance in a porcine‐derived environment
Author(s) -
Wang Mianzhi,
Zeng Zhenling,
Jiang Fengwei,
Zheng Ying,
Shen Huigang,
Macedo Nubia,
Sun Yongxue,
Sahin Orhan,
Li Ganwu
Publication year - 2020
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.15084
Subject(s) - prophage , biology , transposable element , microbiology and biotechnology , genetics , lineage (genetic) , escherichia coli , genome , gene , bacteriophage
Summary Enterotoxigenic Escherichia coli (ETEC) cause acute secretory diarrhoea in pigs, posing a great economic loss to the swine industry. This study analysed the prevalence and genetic characteristics of prophages from 132 ETEC isolates from symptomatic pigs to determine their potential for spreading antibiotic resistance. A total of 1105 potential prophages were identified, and the distribution of the genome size showed three ‘overlapping’ trends. Similarity matrix comparison showed that prophages correlated with the ETEC lineage distribution, and further identification of these prophages corroborated the lineage specificity. In total, 1206 antibiotic resistance genes (ARGs) of 52 different categories were identified in 132 ETEC strains; among these, 2.65% (32/1206) of ARGs were found to be carried by prophages. Analysis of flanking sequences showed that almost all the ARGs could be grouped into two types: ‘ bla TEM‐1B ’ and ‘classic class 1 integron (IntI1)’. They co‐occurred with a strictly conserved recombinase and transposon Tn 3 family but with a difference: the ‘ bla TEM‐1B type’ prophages exhibited a classic Tn 2 transposon structure with 100% sequence identity, whereas the ‘IntI1 type’ co‐occurred with the TnAs 2 transposon with only 84% sequence identity. These results imply that ARGs might be pervasive in natural bacterial populations through transmission by transposable bacteriophages.