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Dynamic expression of intra‐ and extra‐cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration
Author(s) -
Wang Bing,
Yang Jingjing,
Xu Chen,
Yi Lanxing,
Wan Cuihong
Publication year - 2020
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.14931
Subject(s) - biology , proteome , bacteria , nutrient , extracellular , botany , prokaryote , microbiology and biotechnology , biochemistry , ecology , genetics
Summary Nostoc flagelliforme is well known for its strong ecological adaptability in inhabiting desert biological soil crusts. However, the mechanism of its recovery from quiescent to active state after prolonged dormancy remains poorly characterized. Especially how exoproteome be related to the adaptive strategies and participate in the microalgae‐bacteria interaction. In the present work, we analysed the intra‐ and extra‐cellular proteome of N . flagelliforme over a complete rehydration period both in sterilization and in natural condition for the first time. The protein expression profile for N . flagelliforme has more fluctuations during the first 1 h after wetting but been relatively steady after fully hydrated. According to the extracellular proteomic datasets, we found a dynamic secretion of various extracellular hydrolytic enzymes and membrane transport proteins, which were related to peptidoglycan digestion and nutrient exchange respectively. Two‐hundred and thirteen differentially expressed proteins induced by sterilization also reflect variation in nutrient exchange and highlight symbiosis between N . flagelliforme and surrounding bacteria. We also identified 112 phosphopeptides and 217 phosphorylation site of 95 protein of hydrated N . flagelliforme . The time course datasets we present here will be a reference for understanding the molecular processes underlying N . flagelliforme resuscitation and its potential role in microbial community diversification and soil desertification control.