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Carbendazim‐resistance associated β 2 ‐tubulin substitutions increase deoxynivalenol biosynthesis by reducing the interaction between β 2 ‐tubulin and IDH3 in Fusarium graminearum
Author(s) -
Zhou Zehua,
Duan Yabing,
Zhou Mingguo
Publication year - 2020
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.14874
Subject(s) - biology , tubulin , carbendazim , biosynthesis , biochemistry , mutant , microtubule , microbiology and biotechnology , enzyme , fungicide , botany , gene
Summary Microtubule is a well‐known structural protein participating in cell division, motility and vesicle traffic. In this study, we found that β 2 ‐tubulin, one of the microtubule components, plays an important role in regulating secondary metabolite deoxynivalenol (DON) biosynthesis in Fusarium graminearum by interacting with isocitrate dehydrogenase subunit 3 (IDH3). We found IDH3 negatively regulate DON biosynthesis by reducing acetyl‐CoA accumulation in F. graminearum and DON biosynthesis was stimulated by exogenous acetyl‐CoA. In addition, the expression of IDH3 significantly decreased in the carbendazim‐resistant mutant nt167 (Fgβ 2 F167Y ). Furthermore, we found that carbendazim‐resistance associated β 2 ‐tubulin substitutions reducing the interaction intensity between β 2 ‐tubulin and IDH3. Interestingly, we demonstrated that β 2 ‐tubulin inhibitor carbendazim can disrupt the interaction between β 2 ‐tubulin and IDH3. The decreased interaction intensity between β 2 ‐tubulin and IDH3 resulted in the decreased expression of IDH3, which can cause the accumulation of acetyl‐CoA, precursor of DON biosynthesis in F. graminearum . Thus, we revealed that carbendazim‐resistance associated β 2 ‐tubulin substitutions or carbendazim treatment increases DON biosynthesis by reducing the interaction between β 2 ‐tubulin and IDH3 in F. graminearum . Taken together, the novel findings give the new perspectives of β 2 ‐tubulin in regulating secondary metabolism in phytopathogenic fungi.