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A new regulator RsdA mediating fungal secondary metabolism has a detrimental impact on asexual development in Pestalotiopsis fici
Author(s) -
Zhou Shuang,
Zhang Peng,
Zhou Haichuan,
Liu Xingzhong,
Li ShuMing,
Guo Liangdong,
Li Kuan,
Yin WenBing
Publication year - 2019
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.14473
Subject(s) - biology , secondary metabolism , regulator , secondary metabolite , transcriptome , pestalotiopsis , gene , hypha , polyketide synthase , microbiology and biotechnology , melanin , response regulator , regulation of gene expression , transcriptional regulation , genetics , polyketide , gene expression , biosynthesis , botany , mutant
Summary Secondary metabolite (SM) production and development are correlated processes in fungi that are often coordinated by pleiotropic regulators. The eukaryotic regulators are critical players in mediating SM production related to fungal development, yet little data are available to support this hypothesis. In this study, a global regulator, RsdA ( r egulation of s econdary metabolism and d evelopment), was identified through genome‐wide analysis and deletion of the regulator gene in the endophytic fungus Pestalotiopsis fici . Here, we established that RsdA regulation of SMs is accompanied by the repression of asexual development. Deletion of rsdA significantly reduces not only asexual development, resulting in low sporulation and abnormal conidia, but also the major SM production, while remarkably increasing the melanin production. Overproduction of melanin leads to the formation of unusual, heavily pigmented hyphae. Transcriptome analysis data provide the evidence that RsdA globally regulates genes involved in secondary metabolism and asexual development. Double deletion of rsdA and the melanin polyketide synthase gene PfmaE confirm that RsdA regulation of asexual development is independent of the melanin biosynthetic pathway. Finally, our results demonstrate that RsdA can be used for the discovery of secondary metabolites in filamentous fungi.