Premium
2,3‐Butanediol catabolism in Pseudomonas aeruginosa PAO1
Author(s) -
Liu Qiuyuan,
Liu Yidong,
Kang Zhaoqi,
Xiao Dan,
Gao Chao,
Xu Ping,
Ma Cuiqing
Publication year - 2018
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.14332
Subject(s) - acetoin , biology , operon , acetaldehyde , pseudomonas aeruginosa , catabolism , dehydrogenase , biochemistry , metabolite , 2,3 butanediol , microbiology and biotechnology , enzyme , bacteria , fermentation , gene , genetics , escherichia coli , ethanol
Summary 2,3‐Butanediol (2,3‐BD) is a primary microbial metabolite that enhances the virulence of Pseudomonas aeruginosa and alters the lung microbiome. 2,3‐BD exists in three stereoisomeric forms: (2 R ,3 R )‐2,3‐BD, meso ‐2,3‐BD and (2 S ,3 S )‐2,3‐BD. In this study, we investigated whether and how P. aeruginosa PAO1 utilizes these 2,3‐BD stereoisomers and showed that all three stereoisomers were transformed into acetoin by (2 R ,3 R )‐2,3‐butanediol dehydrogenase (BDH) or (2 S ,3 S )‐2,3‐BDH. Acetoin was cleaved to form acetyl‐CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2 R ,3 R )‐2,3‐BDH, (2 S ,3 S )‐2,3‐BDH and the E1 and E2 components of AoDH ES were identified as part of a new 2,3‐BD utilization operon. In addition, the regulatory protein AcoR promoted the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. The results of this study elucidate the integrated catabolic role of 2,3‐BD and may provide new insights in P. aeruginosa‐ related infections.