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Within‐host evolution versus immigration as a determinant of Escherichia coli diversity in the human gastrointestinal tract
Author(s) -
Dixit Ojas V. A.,
O'Brien Claire L.,
Pavli Paul,
Gordon David M.
Publication year - 2018
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.14028
Subject(s) - biology , escherichia coli , genetics , host (biology) , population , single nucleotide polymorphism , strain (injury) , evolutionary biology , microbiology and biotechnology , gene , genotype , demography , anatomy , sociology
Summary When a human host harbors two or more strains of Escherichia coli , the second strain is more likely to be a member of the same phylogroup rather than a different phylogroup. This outcome may be the consequence of a within host evolution event or an independent immigration/establishment event. To determine the relative importance of these two events in determining E. coli diversity in a host, a collection of multiple E. coli isolates recovered from each of 67 patients undergoing colonoscopies was used. Whole genome sequence data were available for one example of every REP‐fingerprint type identified in a patient. Sequence type (ST) and single‐nucleotide polymorphism (SNP) analyses revealed that 83% of strains observed in the host population were a consequence of immigration/establishment events. Restricting the analysis to hosts harboring two or more strains belonging to the same phylogroup revealed that in about half of these cases, the presence of a second strain belonging to the same phylogroup was the consequence of an independent immigration/establishment event. Thus, the results of this study show that despite hosts being exposed to a diversity of E. coli via their food, factors related to the host also determine what E. coli strains succeed in establishing.