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Sorting nexin (MoVps17) is required for fungal development and plant infection by regulating endosome dynamics in the rice blast fungus
Author(s) -
Zheng Huawei,
Guo Zhongkun,
Xi Yang,
Yuan Mingyue,
Lin Yahong,
Wu Congxian,
Abubakar Yakubu Saddeeq,
Dou Xianying,
Li Guangpu,
Wang Zonghua,
Zheng Wenhui,
Zhou Jie
Publication year - 2017
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13896
Subject(s) - biology , sorting nexin , fungus , endosome , sorting , rice plant , botany , microbiology and biotechnology , agronomy , intracellular , computer science , programming language
Summary Vps17 is a sorting nexin (SNX) and a component of the retromer, a protein complex mediating retrograde vesicle transport between endosomes and the trans‐Golgi network. However, its role in the development and pathogenicity of filamentous fungi such as the rice blast fungus ( Magnaporthe oryzae ) remains unclear. We investigate the functional relationship between the SNX and the cargo‐selective complex (CSC) of the fungal retromer by genetic analysis, live cell imaging and immunological assay. Our data show that the MoVps17 null mutation causes defects in growth, development and pathogenicity in M. oryzae . MoVps17 is localized to endosomes depending on the activity of phosphatidylinositol 3‐kinase (PI3K), a key enzyme for fungal development and infection. Both PX and BAR domains of MoVps17 are essential for its endosomal localization and function. Furthermore, our yeast two‐hybrid assays show that MoVps17 and MoVps5 can interact. Lastly, live cell imaging suggests that MoVps17 can regulate early endosome fusion and budding as well as endocytosis. Taken together, our results suggest that MoVps17 specifically functions as a retromer component with CSC and also plays a distinct role in the regulation of endosome dynamics during fungal development and plant infection.