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Methylmalonate‐semialdehyde dehydrogenase mediated metabolite homeostasis essentially regulate conidiation, polarized germination and pathogenesis in Magnaporthe oryzae
Author(s) -
Norvienyeku Justice,
Zhong Zhenhui,
Lin Lili,
Dang Xie,
Chen Meilian,
Lin Xiaolian,
Zhang Honghong,
Anjago Wilfred M.,
Lin Lianyu,
Abdul Waheed,
Wang Zonghua
Publication year - 2017
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13888
Subject(s) - appressorium , biology , conidiation , magnaporthe , germ tube , aldehyde dehydrogenase , mutant , biochemistry , microbiology and biotechnology , fungus , gene , hypha , oryza sativa , magnaporthe grisea , botany
Summary Plants generate multitude of aldehydes under abiotic and biotic stress conditions. Ample demonstrations have shown that rice‐derived aldehydes enhance the resistance of rice against the rice‐blast fungus Magnaporthe oryzae . However, how the fungal pathogen nullifies the inhibitory effects of host aldehydes to establish compatible interaction remains unknown. Here we identified and evaluated the in vivo transcriptional activities of M. oryzae aldehyde dehydrogenase (ALDH) genes. Transcriptional analysis of M. oryzae ALDH genes revealed that the acetylating enzyme Methylmalonate‐Semialdehyde Dehydrogenase (MoMsdh/MoMmsdh) elevated activities during host invasion and colonization of the fungus. We further examined the pathophysiological importance of MoMSDH by deploying integrated functional genetics, and biochemical approaches. MoMSDH deletion mutant Δ Momsdh exhibited germination defect, hyper‐branching of germ tube and failed to form appressoria on hydrophobic and hydrophilic surface. The MoMSDH disruption caused accumulation of small branch‐chain amino acids, pyridoxine and AMP/cAMP in the Δ Momsdh mutant and altered Spitzenkörper organization in the conidia. We concluded that MoMSDH contribute significantly to the pathogenesis of M. oryzae by regulating the mobilization of Spitzenkörper during germ tube morphogenesis, appressoria formation by acting as metabolic switch regulating small branch‐chain amino acids, inositol, pyridoxine and AMP/cAMP homeostasis.