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Effects of different temperature regimes on survival of Diaphorina citri and its endosymbiotic bacterial communities
Author(s) -
Hussain Mubasher,
Akutse Komivi Senyo,
Ravindran Keppanan,
Lin Yongwen,
Bamisile Bamisope Steve,
Qasim Muhammad,
Dash Chandra Kanta,
Wang Liande
Publication year - 2017
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13821
Subject(s) - diaphorina citri , biology , obligate , host (biology) , wolbachia , pest analysis , botany , buchnera , candidatus , zoology , symbiosis , ecology , bacteria , hemiptera , 16s ribosomal rna , genetics
The Asian citrus psyllid, Diaphorina citri , is a major pest of citrus and vector of citrus greening (huanglongbing) in Asian. In our field‐collected psyllid samples, we discovered that Fuzhou (China) and Faisalabad (Pakistan), populations harbored an obligate primary endosymbiont Candidatus Carsonella (gen. nov.) with a single species, Candidatus Carsonella ruddii (sp. nov.) and a secondary endosymbiont, Wolbachia surface proteins (WSP) which are intracellular endosymbionts residing in the bacteriomes. Responses of these symbionts to different temperatures were examined and their host survival assessed. Diagnostic PCR assays showed that the endosymbionts infection rates were not significantly reduced in both D. citri populations after 24 h exposure to cold or heat treatments. Although quantitative PCR assays showed significant reduction of WSP relative densities at 40°C for 24 h, a substantial decrease occurred as the exposure duration increased beyond 3 days. Under the same temperature regimes, Ca. C. ruddii density was initially less affected during the first exposure day, but rapidly reduced at 3–5 days compared to WSP. However, the mortality of the psyllids increased rapidly as exposure time to heat treatment increased. The responses of the two symbionts to unfavorable temperature regimes highlight the complex host‐symbionts interactions between D. citri and its associated endosymbionts.