Premium
Two degradation strategies for overcoming the recalcitrance of natural lignocellulosic xylan by polysaccharides‐binding GH 10 and GH 11 xylanases of filamentous fungi
Author(s) -
Miao Youzhi,
Li Pan,
Li Guangqi,
Liu Dongyang,
Druzhinina Irina S.,
Kubicek Christian P.,
Shen Qirong,
Zhang Ruifu
Publication year - 2017
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13614
Subject(s) - biology , xylan , polysaccharide , microbiology and biotechnology , degradation (telecommunications) , food science , botany , biochemistry , telecommunications , computer science
Summary The recalcitrance of lignocellulose forms a strong barrier for the bioconversion of lignocellulosic biomass in chemical or biofuel industries. Filamentous fungi are major plant biomass decomposer, and capable of forming all the required enzymes. Here, they characterized the GH10 and GH11 endo‐xylanases and a CE1 acetyl‐xylan esterase (Axe1) from a superior biomass‐degrading strain, Aspergillus fumigatus Z5, and examined how they interact in xylan degradation. Cellulose‐binding (CBM1) domain inhibited GH10 xylanase activities for pure xylan, but afforded them an ability to hydrolyze washed corncob particles (WCCP). CBM1‐containing GH10 xylanases also showed synergism with CBM1‐containing Axe1 in WCCP hydrolysis, and this synergy was strictly dependent on the presence of their CBM1 domains. In contrast, GH11 xylanases had no CBM1, but still could bind xylan and hydrolyzed WCCP; however, no synergism displayed with Axe1. GH10 xylanases and GH11 xylanases showed a pronounced synergism in WCCP hydrolysis, which was dependent on the presence of the CBM1 in GH10 xylanases and absence from GH11 xylanases. They exhibit different mechanisms to bind to cellulose and xylan, and act in synergy when these two structures are intact. These findings will be helpful for the further development of highly efficient enzyme mixtures for lignocellulosic biomass conversion.