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Photorhabdus ‐nematode symbiosis is dependent on hfq ‐mediated regulation of secondary metabolites
Author(s) -
Tobias Nicholas J.,
Heinrich Antje K.,
Eresmann Helena,
Wright Patrick R.,
Neubacher Nick,
Backofen Rolf,
Bode Helge B.
Publication year - 2017
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13502
Subject(s) - biology , photorhabdus , photorhabdus luminescens , symbiotic bacteria , symbiosis , xenorhabdus , bacteria , secondary metabolite , quorum sensing , microbiology and biotechnology , mutant , repressor , nematode , genetics , gene , virulence , gene expression , ecology
Summary Photorhabdus luminescens maintains a symbiotic relationship with the nematodes Heterorhabditis bacteriophora and together they infect and kill insect larvae. To maintain this symbiotic relationship, the bacteria must produce an array of secondary metabolites to assist in the development and replication of nematodes. The regulatory mechanisms surrounding production of these compounds are mostly unknown. The global post‐transcriptional regulator, Hfq, is widespread in bacteria and performs many functions, one of which is the facilitation of sRNA binding to target mRNAs, with recent research thoroughly exploring its various pleiotropic effects. Here we generate and characterize an hfq deletion mutant and show that in the absence of hfq , the bacteria are no longer able to maintain a healthy symbiosis with nematodes due to the abolishment of the production of all known secondary metabolites. RNAseq led us to produce a second deletion of a known repressor, HexA, in the same strain, which restored both metabolite production and symbiosis.