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Bacteriophages to reduce gut carriage of antibiotic resistant uropathogens with low impact on microbiota composition
Author(s) -
Galtier Matthieu,
De Sordi Luisa,
Maura Damien,
Arachchi Harindra,
Volant Stevenn,
Dillies MarieAgnès,
Debarbieux Laurent
Publication year - 2016
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13284
Subject(s) - virulence , biology , carriage , microbiology and biotechnology , gut flora , antibiotics , phage therapy , escherichia coli , antibiotic resistance , colonization , virology , bacteriophage , immunology , medicine , genetics , pathology , gene
Summary Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs) worldwide, causing over 150 million clinical cases annually. There is currently no specific treatment addressing the asymptomatic carriage in the gut of UPEC before they initiate UTIs. This study investigates the efficacy of virulent bacteriophages to decrease carriage of gut pathogens. Three virulent bacteriophages infecting an antibiotic‐resistant UPEC strain were isolated and characterized both in vitro and in vivo . A new experimental murine model of gut carriage of E. coli was elaborated and the impact of virulent bacteriophages on colonization levels and microbiota diversity was assessed. A single dose of a cocktail of the three bacteriophages led to a sharp decrease in E. coli levels throughout the gut. We also observed that microbiota diversity was much less affected by bacteriophages than by antibiotics. Therefore, virulent bacteriophages can efficiently target UPEC strains residing in the gut, with potentially profound public health and economic impacts. These results open a new area with the possibility to manipulate specifically the microbiota using virulent bacteriophages, which could have broad applications in many gut‐related disorders/diseases and beyond.