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Alkaline phosphatases are involved in the response of A edes aegypti larvae to intoxication with B acillus thuringiensis subsp. israelensis C ry toxins
Author(s) -
Stalinski Renaud,
Laporte Frédéric,
Després Laurence,
Tetreau Guillaume
Publication year - 2016
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13186
Subject(s) - biology , larva , aedes aegypti , alkaline phosphatase , bacillus thuringiensis , phosphatase , microbiology and biotechnology , food science , biochemistry , phosphorylation , enzyme , ecology , bacteria , genetics
Summary B acillus thuringiensis subsp. israelensis ( B ti) is a natural pathogen of dipterans widely used as a biological insecticide for mosquito control. To characterize the response of mosquitoes to intoxication with B ti, the transcriptome profile of B ti‐exposed susceptible A edes aegypti larvae was analysed using I llumina RNA ‐seq. Gene expression of 11 alkaline phosphatases ( ALPs ) was further investigated by real time quantitative polymerase chain reaction and ALP activity was measured in the susceptible strain and in four strains resistant to a single B ti C ry toxin or to B ti. These strains were unexposed or exposed to their toxin of selection. Although all resistant strains constitutively exhibited a higher level of transcription of ALP genes than the susceptible strain, they showed a lower total ALP activity. The intoxication with different individual C ry toxins triggered a global pattern of ALP gene under‐transcription in all the one‐toxin‐resistant strains but involving different specific sets of ALPs in each resistant phenotype. Most of the ALPs involved are not known C ry‐binding proteins. RNA interference experiment demonstrated that reducing ALP expression conferred increased the survival of larvae exposed to Cry4Aa , confirming the involvement of ALP in Cry4Aa toxicity.