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Impact of OmpR on the membrane proteome of Y ersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR
Author(s) -
Nieckarz Marta,
Raczkowska Adrianna,
Dębski Janusz,
Kistowski Michał,
Dadlez Michał,
Heesemann Jürgen,
Rossier Ombeline,
Brzostek Katarzyna
Publication year - 2016
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13165
Subject(s) - yersinia enterocolitica , biology , psychological repression , bacterial adhesin , yersinia , microbiology and biotechnology , proteome , virulence , heme , transcriptome , gene , gene expression , genetics , bacteria , biochemistry , enzyme
Summary Enteropathogenic Y ersinia enterocolitica is able to grow within or outside the mammalian host. Previous transcriptomic studies have indicated that the regulator OmpR plays a role in the expression of hundreds of genes in enterobacteria. Here, we have examined the impact of OmpR on the production of Y . enterocolitica membrane proteins upon changes in temperature, osmolarity and pH . Proteomic analysis indicated that the loss of OmpR affects the production of 120 proteins, a third of which are involved in uptake/transport, including several that participate in iron or heme acquisition. A set of proteins associated with virulence was also affected. The influence of OmpR on the abundance of adhesin YadA and heme receptor HemR was examined in more detail. OmpR was found to repress YadA production and bind to the yad A promoter, suggesting a direct regulatory effect. In contrast, the repression of hem R expression by OmpR appears to be indirect. These findings provide new insights into the role of OmpR in remodelling the cell surface and the adaptation of Y . enterocolitica to different environmental niches, including the host.