Premium
Analysis of ds DNA and RNA viromes in methanogenic digesters reveals novel viral genetic diversity
Author(s) -
Calusinska Magdalena,
Marynowska Martyna,
Goux Xavier,
Lentzen Esther,
Delfosse Philippe
Publication year - 2016
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13127
Subject(s) - biology , metagenomics , rna , food waste , dna virus , dna , genetic diversity , anaerobic digestion , microbiology and biotechnology , genetics , gene , genome , ecology , population , demography , methane , sociology
Summary Although viruses are not the key players of the anaerobic digestion process, they may affect the dynamics of bacterial and archaeal populations involved in biogas production. Until now viruses have received very little attention in this specific habitat; therefore, as a first step towards their characterization, we optimized a virus filtration protocol from anaerobic sludge. Afterwards, to assess ds DNA and RNA viral diversity in sludge samples from nine different reactors fed either with waste water, agricultural residues or solid municipal waste plus agro‐food residues, we performed metagenomic analyses. As a result we showed that, while the ds DNA viromes (21 assigned families in total) were dominated by ds DNA phages of the order C audovirales , RNA viruses (14 assigned families in total) were less diverse and were for the main part plant‐infecting viruses. Interestingly, less than 2% of annotated contigs were assigned as putative human and animal pathogens. Our study greatly extends the existing view of viral genetic diversity in methanogenic reactors and shows that these viral assemblages are distinct not only among the reactor types but also from nearly 30 other environments already studied, including the human gut, fermented food, deep sea sediments and other aquatic habitats.