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CmpacC regulates mycoparasitism, oxalate degradation and antifungal activity in the mycoparasitic fungus C oniothyrium minitans
Author(s) -
Lou Yi,
Han Yongchao,
Yang Long,
Wu Mingde,
Zhang Jing,
Cheng Jiasen,
Wang Moying,
Jiang Daohong,
Chen Weidong,
Li Guoqing
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.13018
Subject(s) - biology , repressor , mutant , microbiology and biotechnology , saccharomyces cerevisiae , activator (genetics) , oxalate , fungus , transcription factor , biochemistry , gene , botany , chemistry , organic chemistry
Summary The PacC /Rim101 pH ‐responsive transcription factor is an important pathogenicity element for many plant‐pathogenic fungi. In this study, we investigated the roles of a PacC homologue, CmpacC , in the mycoparasitic fungus C oniothyrium minitans . CmpacC was confirmed to have the transcriptional activation activity by the transcriptional activation test in S accharomyces cerevisiae . Disruption of CmpacC resulted in impaired fungal responses to ambient pH . Compared to the wild‐type, the CmpacC ‐disruption mutant Δ CmpacC ‐29 was significantly suppressed for activities of chitinase and β‐1,3‐glucanase at pH 5 and 7, consistent with reduced expression levels of Cmch1 and Cmg1 coding for the two enzymes respectively. However, the mutant displayed acidity‐mimicking phenotypes such as improved oxalate degradation and increased antifungal activity at pH 6 or higher. Improved efficacy in oxalate degradation by Δ CmpacC ‐29 was consistent with the enhanced expression level of Cmoxdc1 coding for oxalate decarboxylase. CmpacC transcriptional activation of Cmch1 and Cmg1 and repression of Cmoxdc1 were verified by the presence of the PacC /Rim101 consensus binding‐motifs in gene promoter regions and by the promoter DNA ‐binding assays. This study suggests that CmpacC plays an activator role in regulation of C . minitans mycoparasitism, whereas plays a repressor role in regulation of oxalate degradation and possibly antifungal activity of C . minitans .