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Histone H3K4 methylation regulates hyphal growth, secondary metabolism and multiple stress responses in F usarium graminearum
Author(s) -
Liu Ye,
Liu Na,
Yin Yanni,
Chen Yun,
Jiang Jinhua,
Ma Zhonghua
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12993
Subject(s) - biology , conidiation , secondary metabolism , mutant , microbiology and biotechnology , histone , histone methylation , methylation , gene , biochemistry , gene expression , dna methylation , biosynthesis
Summary Histone H 3 lysine 4 methylation ( H3K4me ) is generally associated with actively transcribed genes in a variety of eukaryotes. The function of H3K 4me in phytopathogenic fungi remains unclear. Here, we report that F g S et1 is predominantly responsible for mono‐, di‐ and trimethylation of H3K 4 in F usarium graminearum . The FgSET 1 deletion mutant (Δ FgSet 1) was crippled in hyphal growth and virulence. H3 K 4me is required for the active transcription of genes involved in deoxynivalenol and aurofusarin biosyntheses. Unexpectedly, FgSet 1 plays an important role in the response of F . graminearum to cell wall‐damaging agents via negatively regulating phosphorylation of FgMgv 1, a core kinase in the cell wall integrity pathway. In addition, Δ FgSet 1 exhibited increased resistance to the transcription elongation inhibitor mycophenolic acid. Yeast two‐hybrid assays showed that FgSet 1 physically interacts with multiple proteins including FgBre 2, FgSpp 1 and FgSwd 2. FgBre 2 further interacts with FgSdc 1. Western blotting analyses showed that FgBre 2 and FgSdc 1 are associated with H3K4me . Both proteins are also involved in regulating deoxynivalenol biosynthesis and in responses to mycophenolic acid and cell wall‐damaging agents. Taken together, these data indicate that H 3 K 4me plays critical roles not only in regulation of fungal growth and secondary metabolism but also in multiple stress responses in F . graminearum .