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Rab GTP ases are essential for membrane trafficking‐dependent growth and pathogenicity in F usarium graminearum
Author(s) -
Zheng Huawei,
Zheng Wenhui,
Wu Congxian,
Yang Jie,
Xi Yang,
Xie Qiurong,
Zhao Xu,
Deng Xiaolong,
Lu Guodong,
Li Guangpu,
Ebbole Daniel,
Zhou Jie,
Wang Zonghua
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12982
Subject(s) - rab , biology , gtpase , endocytic cycle , microbiology and biotechnology , vacuole , endocytosis , exocytosis , cytoplasm , biochemistry , cell , membrane
Summary Rab GTPases represent the largest subfamily of Ras‐related small GTP ases and regulate membrane trafficking. Vesicular transport is a general mechanism that governs intracellular membrane trafficking along the endocytic and exocytic pathways in all eukaryotic cells. F usarium graminearum is a filamentous fungus and causes the devastating and economically important head blight of wheat and related species. The mechanism of vesicular transport is not well understood, and little is known about Rab GTP ases in F . graminearum . In this study, we systematically characterized all eleven FgRabs by live cell imaging and genetic analysis. We find that FgRab51 and FgRab52 are important for the endocytosis, FgR ab7 localizes to the vacuolar membrane and regulates the fusion of vacuoles and autophagosomes, and FgR ab8 and FgRab11 are important for polarized growth and/or exocytosis. Furthermore, both endocytic and exocytic FgR abs are required for vegetative growth, conidiogenesis, sexual reproduction, as well as pathogenesis and deoxynivalenol metabolism in F . graminearum . Thus, we conclude that R ab GTP ases are essential for membrane trafficking‐dependent growth and pathogenicity in F . graminearum .