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A novel protein quality control mechanism contributes to heat shock resistance of worldwide‐distributed P seudomonas aeruginosa clone C strains
Author(s) -
Lee Changhan,
Wigren Edvard,
Trček Janja,
Peters Verena,
Kim Jihong,
Hasni Muhammad Sharif,
Nimtz Manfred,
Lindqvist Ylva,
Park Chankyu,
Curth Ute,
Lünsdorf Heinrich,
Römling Ute
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12915
Subject(s) - biology , clone (java method) , heat shock protein , operon , pseudomonas aeruginosa , microbiology and biotechnology , heat shock , genomic island , shock (circulatory) , chaperone (clinical) , gene , genetics , bacteria , genome , escherichia coli , medicine , pathology
Summary P seudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P . aeruginosa clone C ‐specific genomic island ( PACGI ‐1) that contains the highly expressed small heat shock protein sHsp 20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp 20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp 20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp 20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere‐like 24‐meric oligomers and exhibits significant chaperone activity. As the P . aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P . aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide‐distributed clone C strains.