Premium
Single‐cell genomics of uncultivated deep‐branching magnetotactic bacteria reveals a conserved set of magnetosome genes
Author(s) -
Kolinko Sebastian,
Richter Michael,
Glöckner FrankOliver,
Brachmann Andreas,
Schüler Dirk
Publication year - 2016
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12907
Subject(s) - magnetosome , biology , candidatus , genome , metagenomics , phylum , proteobacteria , chloroflexi (class) , comparative genomics , genetics , ingression , bacterial genome size , genomics , gene , bacteria , 16s ribosomal rna , gastrulation , embryonic stem cell
Summary While magnetosome biosynthesis within the magnetotactic P roteobacteria is increasingly well understood, much less is known about the genetic control within deep‐branching phyla, which have a unique ultrastructure and biosynthesize up to several hundreds of bullet‐shaped magnetite magnetosomes arranged in multiple bundles of chains, but have no cultured representatives. Recent metagenomic analysis identified magnetosome genes in the genus ‘ C andidatus M agnetobacterium’ homologous to those in P roteobacteria . However, metagenomic analysis has been limited to highly abundant members of the community, and therefore only little is known about the magnetosome biosynthesis, ecophysiology and metabolic capacity in deep‐branching MTB . Here we report the analysis of single‐cell derived draft genomes of three deep‐branching uncultivated MTB . Single‐cell sorting followed by whole genome amplification generated draft genomes of C andidatus M agnetobacterium bavaricum and C andidatus M agnetoovum chiemensis CS ‐04 of the N itrospirae phylum. Furthermore, we present the first, nearly complete draft genome of a magnetotactic representative from the candidate phylum O mnitrophica, tentatively named C andidatus O mnitrophus magneticus SKK ‐01. Besides key metabolic features consistent with a common chemolithoautotrophic lifestyle, we identified numerous, partly novel genes most likely involved in magnetosome biosynthesis of bullet‐shaped magnetosomes and their arrangement in multiple bundles of chains.