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Unveiling equal importance of two 14‐3‐3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen
Author(s) -
Liu Qian,
Li JinGen,
Ying ShengHua,
Wang JuanJuan,
Sun WenLiang,
Tian ChaoGuang,
Feng MingGuang
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12634
Subject(s) - conidiation , biology , virulence , morphogenesis , pathogen , insect , microbiology and biotechnology , genetics , gene , ecology
Summary Two conserved 14‐3‐3 proteins orthologous to S accharomyces cerevisiae Bmh 1/2 are poorly understood in filamentous fungi. Here we show that Bmh 1 and Bmh 2 contribute equally to the fundamental biology and physiology of B eauveria bassiana by targeting many sets of proteins/enzymes. Single Bmh deletion caused similar upregulation of another. Excellent knockdown (∼91%) expressions of Bmh 1 in Δ Bmh 2 and Bmh 2 in Δ Bmh 1 resulted in equally more severe multiphenotypic defects than the single deletions, including G 2 / M transition, blastospore size, carbon/nitrogen utilization, conidiation, germination and conidial tolerances to high osmolarity, oxidation, cell wall stress, high temperature and UV ‐ B irradiation. All the deletion and deletion/knockdown mutants showed similar defects in blastospore yield and density, hyphal septation and cell size, hyphal responses to most chemical stresses and virulence. All the defects were evident with altered transcripts of phenotype‐related genes and well restored by each Bmh complementation. Our Bmh 1 ‐ and Bmh 2 ‐specific transcriptomes generated under osmotic and oxidative stresses revealed up to 6% genes differentially expressed by at least twofold in the fungal genome. Many of those were greatly depressed or co‐depressed in Δ Bmh 1 and Δ Bmh 2 . Our findings provide a thorough insight into the functions and complementary effects of the two 14‐3‐3 proteins in the filamentous entomopathogen.