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Insights into organohalide respiration and the versatile catabolism of S ulfurospirillum multivorans gained from comparative genomics and physiological studies
Author(s) -
Goris Tobias,
Schubert Torsten,
Gadkari Jennifer,
Wubet Tesfaye,
Tarkka Mika,
Buscot Francois,
Adrian Lorenz,
Diekert Gabriele
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12589
Subject(s) - biology , genome , gene , bacteria , biochemistry , catabolism , genetics , microbiology and biotechnology , enzyme
Summary S ulfurospirillum multivorans , a free‐living ε‐proteobacterium, is among the best studied organisms capable of organohalide respiration. It is able to use several halogenated ethenes as terminal electron acceptor. In this report, the complete genome sequence of S . multivorans including a comparison with genome sequences of two related non‐dehalogenating species, S ulfurospirillum deleyianum and S ulfurospirillum barnesii , is described. The 3.2 Mbp genome of S . multivorans revealed a ∼ 50 kbp gene region encoding proteins required for organohalide respiration and corrinoid cofactor biosynthesis. This region includes genes for components not detected before in organohalide‐respiring organisms. A transcript analysis of genes coding for some of these proteins indicates the involvement of a putative quinol dehydrogenase in organohalide respiration. The presence of genes encoding a variety of oxidoreductases reflects the organism's metabolic versatility. This was confirmed by growth studies with different electron acceptors including perchlorate and several sulfur‐containing compounds. A comparison with other ε‐proteobacteria indicates horizontal acquisition of many genes in the S . multivorans genome, which might be the basis of the bacterium's catabolic flexibility.