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FgSKN 7 and FgATF 1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in F usarium graminearum
Author(s) -
Jiang Cong,
Zhang Shijie,
Zhang Qiang,
Tao Yin,
Wang Chenfang,
Xu JinRong
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12561
Subject(s) - biology , mutant , conidiation , virulence , phenotype , reactive oxygen species , oxidative stress , microbiology and biotechnology , gene , genetics , biochemistry
Summary Fusarium head blight caused by F usarium graminearum is one of the most destructive diseases of wheat and barley. Deoxynivalenol ( DON ) produced by the pathogen is an important mycotoxins and virulence factor. Because oxidative burst is a common defense response and reactive oxygen species ( ROS ) induces DON production, in this study, we characterized functional relationships of three stress‐related transcription factor genes FgAP 1 , FgATF 1 and FgSKN 7. Although all of them played a role in tolerance to oxidative stress, deletion of FgAP 1 or FgATF 1 had no significant effect on DON production. In contrast, F gskn7 mutants were reduced in DON production and defective in H 2 O 2 ‐induced TRI gene expression. The F gap1 mutant had no detectable phenotype other than increased sensitivity to H 2 O 2 and F gap1 F gatf1 and F gap1 F gskn7 mutants lacked additional or more severe phenotypes than the single mutants. The F gatf1 , but not F gskn7 , mutant was significantly reduced in virulence and delayed in ascospore release. The F gskn7 F gatf1 double mutant had more severe defects in growth, conidiation and virulence than the F gatf1 or F gskn7 mutant. Instead of producing four‐celled ascospores, it formed eight small, single‐celled ascospores in each ascus. Therefore, FgSKN 7 and FgATF 1 must have overlapping functions in intracellular ROS signalling for growth, development and pathogenesis in F . graminearum .