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A single exposure to a sublethal pediocin concentration initiates a resistance‐associated temporal cell envelope and general stress response in L isteria monocytogenes
Author(s) -
Laursen Martin F.,
Bahl Martin I.,
Licht Tine R.,
Gram Lone,
Knudsen Gitte M.
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12534
Subject(s) - bacteriocin , listeria monocytogenes , biology , cell envelope , microbiology and biotechnology , gene , bacteria , transcriptome , mutant , sigma factor , gene expression , genetics , escherichia coli , promoter
Summary L isteria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L . monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin‐containing L actobacillus plantarum WHE 92 supernatant could prime L . monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180 min of exposure to L . plantarum WHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL . Additionally, several genes involved in bacteriocin resistance were induced. Δ lisR , Δ sigB and Δ sigL mutants were all more resistant than wild types to L . plantarum WHE 92 supernatant. Conclusively, LisRK , SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed.