Premium
Genome‐scale metabolic reconstruction and constraint‐based modelling of the Antarctic bacterium P seudoalteromonas haloplanktis TAC 125
Author(s) -
Fondi Marco,
Maida Isabel,
Perrin Elena,
Mellera Alessandra,
Mocali Stefano,
Parrilli Ermenegilda,
Tutino Maria Luisa,
Liò Pietro,
Fani Renato
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12513
Subject(s) - biology , phenomics , in silico , metabolic network , computational biology , genome , model organism , metabolic pathway , gene , bacteria , proteomics , genomics , genetics
Summary The A ntarctic strain P seudoalteromonas haloplanktis TAC 125 is one of the model organisms of cold‐adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Here, we investigated several metabolic features of this strain through in silico modelling and functional integration of –omics data. A genome‐scale metabolic model of P . haloplanktis TAC 125 was reconstructed, encompassing information on 721 genes, 1133 metabolites and 1322 reactions. The predictive potential of this model was validated against a set of experimentally determined growth rates and a large dataset of growth phenotypic data. Furthermore, evidence synthesis from proteomics, phenomics, physiology and metabolic modelling data revealed possible drawbacks of cold‐dependent changes in gene expression on the overall metabolic network of P . haloplanktis TAC 125. These included, for example, variations in its central metabolism, amino acid degradation and fatty acid biosynthesis. The genome‐scale metabolic model described here is the first one reconstructed so far for an A ntarctic microbial strain. It allowed a system‐level investigation of variations in cellular metabolic fluxes following a temperature downshift. It represents a valuable platform for further investigations on P . haloplanktis TAC 125 cellular functional states and for the design of more focused strategies for its possible biotechnological exploitation.