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Tolerance to acetic acid is improved by mutations of the TATA ‐binding protein gene
Author(s) -
An Jieun,
Kwon Hyeji,
Kim Eunjung,
Lee Young Mi,
Ko Hyeok Jin,
Park Hongjae,
Choi InGeol,
Kim Sooah,
Kim Kyoung Heon,
Kim Wankee,
Choi Wonja
Publication year - 2015
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12489
Subject(s) - acetic acid , biology , mutant , amino acid , biochemistry , gene , microbiology and biotechnology
Summary Screening a library of overexpressing mutant alleles of the TATA ‐binding gene SPT15 yielded two S accharomyces cerevisiae strains ( MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress‐ and protein synthesis‐related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid‐tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δ mutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.