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Diet alters probiotic L actobacillus persistence and function in the intestine
Author(s) -
Tachon Sybille,
Lee Bokyung,
Marco Maria L.
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12297
Subject(s) - biology , probiotic , lactobacillus plantarum , feces , microbiology and biotechnology , gut flora , persistence (discontinuity) , bacteria , medicine , immunology , lactic acid , genetics , geotechnical engineering , engineering
Summary We investigated the effects of host diet on the intestinal persistence and gene expression of L actobacillus plantarum WCFS 1 in healthy and health‐compromised, 2,4,6‐trinitrobenzene sulfonic acid ( TNBS )‐treated mice. Mice fed either a low‐fat chow diet ( CD ) or high fat and sucrose Western diet ( WD ) received 10 9 L . plantarum WCFS 1 cells for five consecutive days. L actobacillus plantarum persistence was 10‐ to 100‐fold greater in the intestines of WD ‐fed compared with CD ‐fed mice. TNBS , an intestinal irritant that induces the development of inflammatory bowel disease‐like symptoms, resulted in up to a 10 4 ‐fold increase in L . plantarum survival in the digestive tract relative to healthy animals. Expression levels of 12 metabolic and gut‐inducible L . plantarum genes were differentially affected by diet and TNBS administration. Pyrosequencing of 16 S rRNA transcripts from the indigenous intestinal microbiota showed that WD resulted in significant reductions in proportions of metabolically active indigenous L actobacillus species and increases in the Desulfovibrionaceae family. Feeding L . plantarum WCFS 1 resulted in lower levels of colitis and higher concentrations of colonic IL ‐10 and IL ‐12 in WD and not CD ‐fed mice. Interactions between probiotics, nutritional components and the intestinal bacteria should be considered when examining for probiotic‐mediated effects and elucidating mechanisms of probiotic function in the mammalian gut.