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Seasonally variable intestinal metagenomes of the red palm weevil ( R hynchophorus ferrugineus )
Author(s) -
Jia Shangang,
Zhang Xiaowei,
Zhang Guangyu,
Yin An,
Zhang Sun,
Li Fusen,
Wang Lei,
Zhao Duojun,
Yun Quanzheng,
Wang Jixiang,
Sun Gaoyuan,
Baabdullah Mohammed,
Yu Xiaoguang,
Hu Songnian,
AlMssallem Ibrahim S.,
Yu Jun
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12262
Subject(s) - biology , rhynchophorus , weevil , pyrosequencing , palm , 16s ribosomal rna , pest analysis , gut flora , biological pest control , lactococcus , microbiome , microbiology and biotechnology , zoology , botany , bacteria , lactococcus lactis , lactic acid , biochemistry , physics , genetics , quantum mechanics , immunology , gene , bioinformatics
Summary The intestinal microbes residing in the red palm weevil ( RPW , R hynchophorus ferrugineus ) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months ( M arch, J uly and N ovember), and then confirm the impact of high‐temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify K lebsiella pneumoniae in N ovember and L actococcus lactis in J uly as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in N ovember and J uly are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization.