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Membrane vesicle formation is associated with pyocin production under denitrifying conditions in P seudomonas aeruginosa PAO 1
Author(s) -
Toyofuku Masanori,
Zhou Shengmin,
Sawada Isao,
Takaya Naoki,
Uchiyama Hiroo,
Nomura Nobuhiko
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12260
Subject(s) - denitrifying bacteria , biology , quorum sensing , bacteria , anoxic waters , microbiology and biotechnology , nitric oxide , mutant , biofilm , biochemistry , chemistry , gene , genetics , ecology , denitrification , organic chemistry , nitrogen , endocrinology
Summary Many G ram‐negative bacteria produce membrane vesicles ( MVs ) that serve as vehicles to mediate intraspecies and interspecies interactions. Despite their ubiquity in G ram‐negative bacteria and their biological importance, how MV formation is regulated is poorly understood. P seudomonas aeruginosa is a ubiquitous bacterium that is one of the most extensively studied model organism in MVs . Recent studies highlight the importance of a quorum‐sensing signal, P seudomonas quinolone signal ( PQS ), in the formation of MVs ; however, PQS synthesis requires oxygen and is not produced under anoxic conditions. This situation leads to the question of MV production under anoxic conditions. Here, we examined whether MVs are produced under denitrifying conditions and what kind of factors are involved in the MV production under such condition. Under denitrifying condition, P . aeruginosa PAO 1 produced a considerable amount of MVs . Interestingly, pyocin components were found to be accumulated in the isolated MVs . Pyocin‐related protein mutants produced less MVs compared with the wild type. We further indicate that pyocin production is activated by nitric oxide, in which the SOS response is involved. This study presents a regulatory mechanism where pyocin is associated with MV production, and further implies how the environment impacts MV production in P . aeruginosa .