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Sulphur‐oxidizing and sulphate‐reducing communities in B razilian mangrove sediments
Author(s) -
VaronLopez Maryeimy,
Dias Armando Cavalcante Franco,
Fasanella Cristiane Cipolla,
Durrer Ademir,
Melo Itamar Soares,
Kuramae Eiko Eurya,
Andreote Fernando Dini
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12237
Subject(s) - mangrove , biology , temperature gradient gel electrophoresis , microbial population biology , library , bacteria , ecology , soil microbiology , environmental chemistry , soil water , 16s ribosomal rna , chemistry , genetics
Summary Mangrove soils are anaerobic environments rich in sulphate and organic matter. Although the sulphur cycle is one of the major actors in this ecosystem, little is known regarding the sulphur bacteria communities in mangrove soils. We investigated the abundance, composition and diversity of sulphur‐oxidizing ( SOB ) and sulphate‐reducing ( SRB ) bacteria in sediments from three B razilian mangrove communities: two contaminated, one with oil ( OilMgv ) and one with urban waste and sludge ( AntMgv ), and one pristine ( PrsMgv ). The community structures were assessed using quantitative real‐time polymerase chain reaction ( qPCR ), polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) and clone libraries, using genes for the enzymes adenosine‐5′‐phosphosulphate reductase ( aprA ) and sulphite reductase ( D sr) ( dsrB ). The abundance for qPCR showed the ratio dsrB/aprA to be variable among mangroves and higher according to the gradient observed for oil contamination in the OilMgv . The PCR‐DGGE patterns analysed by N onmetric M ultidimensional S caling revealed differences among the structures of the three mangrove communities. The clone libraries showed that B etaproteobacteria , G ammaproteobacteria and D eltaproteobacteria were the most abundant groups associated with sulphur cycling in mangrove sediments. We conclude that the microbial SOB and SRB communities in mangrove soils are different in each mangrove forest and that such microbial communities could possibly be used as a proxy for contamination in mangrove forests.