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Identification of a putative cognate sensor kinase for the two‐component response regulator HrpG , a key regulator controlling the expression of the hrp genes in X anthomonas campestris pv. campestris
Author(s) -
Li RuiFang,
Lu GuangTao,
Li Lei,
Su HuiZhao,
Feng Guofang,
Chen Ya,
He YongQiang,
Jiang BoLe,
Tang DongJie,
Tang JiLiang
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12207
Subject(s) - response regulator , biology , regulator , hypersensitive response , virulence , open reading frame , xanthomonas campestris , gene , kinase , microbiology and biotechnology , genetics , mutant , peptide sequence , plant disease resistance
Summary The bacterial phytopathogen X anthomonas campestris pv. campestris ( Xcc ) relies on the hrp (hypersensitive response and pathogenicity) genes to cause disease and induce hypersensitive response ( HR ). The hrp genes of bacterial phytopathogens are divided into two groups. Xcc hrp genes belong to group II . It has long been known that the group II hrp genes are activated by an AraC ‐type transcriptional regulator whose expression is controlled by a two‐component system ( TCS ) response regulator (named HrpG in Xcc ). However, no cognate sensor kinase has yet been identified. Here, we present evidence showing that the Xcc open‐reading frame XC _3670 encodes a TCS sensor kinase (named HpaS ). Mutation of hpaS almost completely abolished the HR induction and virulence. Bacterial two‐hybrid and protein pull‐down assays revealed that HpaS physically interacted with HrpG . Phos‐tag™ SDS‐PAGE analysis showed that mutation in hpaS reduced markedly the phosphorylation of HrpG in vivo . These data suggest that HpaS and HrpG are most likely to form a TCS . We also showed that XC _3669 (named hpaR2 ), which is adjacent to hpaS and encodes a putative TCS response regulator, is required for full virulence but not HR induction. HpaR2 also physically interacted with HpaS , suggesting that HpaS may also form another TCS with HpaR2 .