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Mild gut inflammation modulates the proteome of intestinal E scherichia coli
Author(s) -
Schumann Sara,
Alpert Carl,
Engst Wolfram,
Klopfleisch Robert,
Loh Gunnar,
Bleich André,
Blaut Michael
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12192
Subject(s) - lysozyme , escherichia coli , biology , proinflammatory cytokine , probiotic , microbiology and biotechnology , proteome , inflammation , messenger rna , downregulation and upregulation , intestinal mucosa , bacteria , biochemistry , immunology , gene , medicine , genetics
Summary Using interleukin 10‐deficient ( IL ‐10 ‐/‐ ) and wild‐type mice monoassociated with either the adherent‐invasive E scherichia coli UNC or the probiotic E . coli N issle, the effect of a mild intestinal inflammation on the bacterial proteome was studied. Within 8 weeks, IL ‐10 ‐/‐ mice monoassociated with E . coli UNC exhibited an increased expression of several proinflammatory markers in caecal mucosa. E scherichia coli N issle‐associated IL ‐10 ‐/‐ mice did not do so. As observed previously for E . coli from mice with acute colitis, glycolytic enzymes were downregulated in intestinal E . coli UNC from IL ‐10 ‐/‐ mice. In addition, the inhibitor of vertebrate C ‐type lysozyme, I vy, was upregulated on messenger RNA ( mRNA ) and protein level in E . coli N issle from IL ‐10 ‐/‐ mice compared with E . coli UNC from these mice. Higher expression of I vy in E . coli N issle correlated with an improved growth of this probiotic strain in the presence of lysozyme‐ethylenediaminetetraacetic acid ( EDTA ). By overexpressing I vy, we demonstrated that I vy contributes to a higher lysozyme resistance of E . coli , supporting the role of I vy as a potential fitness factor. However, deletion of I vy did not alter the growth phenotype of E . coli N issle in the presence of lysozyme‐ EDTA , suggesting the existence of additional lysozyme inhibitors that can take over the function of Ivy.