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Targeting spatiotemporal dynamics of planktonic SAGMGC ‐1 and segregation of ammonia‐oxidizing thaumarchaeota ecotypes by newly designed primers and quantitative polymerase chain reaction
Author(s) -
RestrepoOrtiz Claudia X.,
Auguet JeanChristophe,
Casamayor Emilio O.
Publication year - 2014
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12191
Subject(s) - ecotype , biology , thaumarchaeota , archaea , 16s ribosomal rna , ecology , botany , gene , genetics
Summary The annual dynamics of three different ammonia‐oxidizing archaea ( AOA ) ecotypes ( amo A gene) and of the SAGMGC ‐1 ( N itrosotalea ‐like aquatic T haumarchaeota) group (16 S rRNA gene) were studied by newly designed specific primers and quantitative polymerase chain reaction analysis in a deep oligotrophic high mountain lake ( L ake R edon, L imnological O bservatory of the P yrenees, S pain). We observed segregated distributions of the main AOA populations, peaking separately in time and space, and under different ammonia concentrations and irradiance conditions. Strong positive correlation in gene abundances was found along the annual survey between 16 S rRNA SAGMAGC ‐1 and one of the amo A ecotypes suggesting the potential for ammonia oxidation in the freshwater SAGMAGC ‐1 clade. We also observed dominance of N itrosotalea ‐like ecotypes over N itrosopumilus ‐like ( M arine G roup 1.1a) and not the same annual dynamics for the two thaumarchaeotal clades. The fine scale segregation in space and time of the different AOA ecotypes indicated the presence of phylogenetically close but ecologically segregated AOA species specifically adapted to specific environmental conditions. It remains to be elucidated what would be such environmental drivers.