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The tetraspanin gene MaPls1 contributes to virulence by affecting germination, appressorial function and enzymes for cuticle degradation in the entomopathogenic fungus, M etarhizium acridum
Author(s) -
Luo Sha,
He Min,
Cao Yueqing,
Xia Yuxian
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12166
Subject(s) - appressorium , biology , tetraspanin , microbiology and biotechnology , hypha , entomopathogenic fungus , mutant , wild type , gene , botany , biochemistry , cell , beauveria bassiana , biological pest control
Summary In most eukaryotes, tetraspanins regulate cellular activities by associating with other membrane components. In phytopathogenic fungi, the tetraspanin Pls1 controls appressorium‐mediated penetration. However, regulation of Pls1 and its associated signalling pathways are not clear. In this study, the MaPls1 gene from the entomopathogenic fungus M etarhizium acridum was functionally characterized. MaPls1 was highly expressed in mycelium and appressorium, and accumulated on the plasma membrane or in the cytoplasm. Compared with a wild‐type strain, the deletion mutant Δ MaPls1 had delayed germination and appressorium formation and impaired turgor pressure on locust wings, but normal germination on medium and non‐host insect matrices. Bioassays showed that Δ MaPls1 had decreased virulence and hyphal body formation in haemolymph when topically inoculated, but was not different from wild type when the insect cuticle was bypassed. Moreover, the ability to grow out of the cuticle was impaired in Δ MaPls1 . Digital gene expression profiling revealed that genes involved in hydrolysing host cuticle and cell wall synthesis and remodelling were downregulated in Δ MaPls1 . MaPls1 participated in crosstalk with signalling pathways such as the cyclic adenosine monophosphate‐dependent protein kinase A and calmodulin‐dependent pathways. Taken together, these results demonstrated the important roles of MaPls1 at the early stage of infection‐associated development in M . acridum .