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RubisCO ‐based CO 2 fixation and C 1 metabolism in the actinobacterium P seudonocardia dioxanivorans CB 1190
Author(s) -
Grostern Ariel,
AlvarezCohen Lisa
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12144
Subject(s) - biology , autotroph , rubisco , carbon fixation , biochemistry , bacteria , botany , microbiology and biotechnology , photosynthesis , genetics
Summary P seudonocardia is an actinobacterial genus of interest due to its potential biotechnological, medical and environmental remediation applications, as well as for the ecologically relevant symbiotic relationships it forms with attine ants. Some P seudonocardia spp. can grow autotrophically, but the genetic basis of this capability has not previously been reported. I n this study, we examined autotrophy in P seudonocardia dioxanivorans CB 1190, which can grow using H 2 and CO 2 , as well as heterotrophically. Genomic and transcriptomic analysis of CB 1190 cells grown with H 2 /bicarbonate implicated the C alvin– B enson– B assham ( CBB ) cycle in growth‐supporting CO 2 fixation, as well as a [ NiFe ] hydrogenase‐encoding gene cluster in H 2 oxidation. The CBB cycle genes are evolutionarily most related to actinobacterial homologues, although synteny has not been maintained. Ribulose‐1,5‐bisphosphate carboxylase activity was confirmed in H 2 /bicarbonate‐grown CB 1190 cells and was detected in cells grown with the C 1 compounds formate, methanol and carbon monoxide. We also demonstrated the upregulation of CBB cycle genes upon exposure of CB 1190 to these C 1 substrates, and identified genes putatively involved in generating CO 2 from the C 1 substrates by using RT ‐ qPCR . Finally, the potential for autotrophic growth of other P seudonocardia spp. was explored, and the ecological implications of autotrophy in attine ant‐ and plant root‐associated P seudonocardia discussed.