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Involvement of two type 2 C protein phosphatases B c P tc1 and B c P tc3 in the regulation of multiple stress tolerance and virulence of B otrytis cinerea
Author(s) -
Yang Qianqian,
Jiang Jinhua,
Mayr Christiane,
Hahn Matthias,
Ma Zhonghua
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12126
Subject(s) - complementation , biology , mutant , conidiation , virulence , wild type , phosphatase , yeast , saccharomyces cerevisiae , gene , phosphorylation , microbiology and biotechnology , biochemistry
Summary Type 2 C S er/ T hr phosphatases ( PP2Cs ) are involved in various cellular processes in many eukaryotes, but little has been known about their functions in filamentous fungi. B otrytis cinerea contains four putative PP2C genes, named BcPTC1 , ‐ 3 , ‐ 5 , and ‐ 6 . Biological functions of these genes were analysed by gene deletion and complementation. While no phenotypes aberrant from the wild type were observed with mutants of BcPTC5 and BcPTC6 , mutants of BcPTC1 and BcPTC3 had reduced hyphal growth, increased conidiation, and impaired sclerotium development. Additionally, BcPTC1 and BcPTC3 mutants exhibited increased sensitivity to osmotic and oxidative stresses, and to cell wall degrading enzymes. Both mutants exhibited dramatically decreased virulence on host plant tissues. All of the defects were restored by genetic complementation of the mutants with wild‐type BcPTC1 and BcPTC3 respectively. Different from what is known in S accharomyces cerevisiae , B c P tc3, but not B c P tc1, negatively regulates phosphorylation of B c S ak1 (the homologue of S . cerevisiae H og1) in B . cinerea , although both BcPTC1 and BcPTC3 were able to rescue the growth defects of a yeast PTC1 deletion mutant under various stress conditions. These results demonstrated that B c P tc1 and B c P tc3 play important roles in the regulation of multiple stress tolerance and virulence of B . cinerea.