Premium
Characterization of water and wildlife strains as a subgroup of C ampylobacter jejuni using DNA microarrays
Author(s) -
Stabler Richard A.,
Larsson Jonas T.,
AlJaberi Suaad,
Nielsen Eva M.,
Kay Emily,
Tam Clarence C.,
Higgins Craig D.,
Rodrigues Laura C.,
Richardson Judith F.,
O'Brien Sarah J.,
Wren Brendan W.
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/1462-2920.12111
Subject(s) - biology , dna microarray , microbiology and biotechnology , dna , campylobacter jejuni , bacteria , computational biology , genetics , gene , gene expression
Summary C ampylobacter jejuni is the leading cause of human bacterial gastroenteritis worldwide, but source attribution of the organism is difficult. Previously, DNA microarrays were used to investigate isolate source, which suggested a non‐livestock source of infection. In this study we analysed the genome content of 162 clinical, livestock and water and wildlife ( WW ) associated isolates combined with the previous study. Isolates were grouped by genotypes into nine clusters ( C 1 to C 9). Multilocus sequence typing ( MLST ) data demonstrated that livestock associated clonal complexes dominated clusters C 1– C 6. The majority of WW isolates were present in the C 9 cluster. Analysis of previously reported genomic variable regions demonstrated that these regions were linked to specific clusters. Two novel variable regions were identified. A six gene multiplex PCR ( mPCR ) assay, designed to effectively differentiated strains into clusters, was validated with 30 isolates. A further five WW isolates were tested by m PCR and were assigned to the C 7‐ C 9 group of clusters. The predictive mPCR test could be used to indicate if a clinical case has come from domesticated or WW sources. Our findings provide further evidence that WW C . jejuni subtypes show niche adaptation and may be important in causing human infection.